Part:BBa_K2323004:Design

Lwa Cas13a under T7 promoter with solubility tag

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1768
  Illegal BglII site found at 3448
  Illegal BamHI site found at 144
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2847
  

Design Notes

Cas proteins are not easily soluble in E.coli, as they are large. They need to be tagged with a solubility tag, that should be cleaved afterwards.

Source

Leptotrichia wadei